But these methods are associated with high sludge formation, high cost, generation of unwanted byproducts and secondary pollution. Physio-chemical techniques, for example, coagulation-flocculation, adsorption, oxidation and membrane techniques have been used for decoloring of textile dyes during the past several decades. In Brazil, several textile dyes that contaminate drinking water source have been reported to be carcinogenic. In addition, evaporation of these dyes and breathing them cause various allergic reactions and also hazardous for children. Because of their non-degradable nature, these dyes inhibit the entrance sunlight in water and hamper the photosynthesis process, thus affecting the aquatic flora and fauna. One million tons of synthetic dyes are produced each year worldwide and approximately 280,000 tons of these dyes are emitted to the industrial effluents annually. Textile industries besides having a contribution to the development of the global economy, water pollution by textile effluents is one of the major concerned issues over the world. These findings indicate that the two potential bacterial strains can be used in large scale treatment of textile effluents in the future. The two bacterial strains CV-S1 and CM-S1 showed complete Malachite Green dye degradation up to 15 mg/l under shaking condition with 5% (v/v) inoculums at pH 6.50 and temperature 35☌ within 72 and 144 hours respectively. The RAPD analysis and 16S rRNA sequencing confirmed the genetical difference of the isolated strains Enterobacter sp. (CV-S1 and CM-S1) were isolated from textile effluents showing potential degradation efficiency against Malachite Green dye. Two competent bacterial strains of Enterobacter spp. A photo-electric-colorimeter was used to measure the decolorizing activity of bacteria at different time intervals after aerobic incubation. Different growth and culture parameters such as temperature, pH, inoculum-size and dye concentration were optimized to perform the dye-degradation assay using different concentrations of Malachite Green dye in mineral salt medium. This study aimed to isolate and identify potential Malachite Green dye degrading bacteria from textile effluents. Malachite Green dye of the triphenylmethane group is a key component of textile effluents. Water pollution from textile effluent is now one of the major issues all over the world.
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